HPLC法测定复方血竭凝胶中阿魏酸的含量 |
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作者:刘超英,刘丹,张小燕,韩建伟
【摘要】 目的 建立复方血竭凝胶中阿魏酸的含量测定方法。方法 采用高效液相色谱(HPLC)法,色谱柱为ZORBAX SB-C18(5 μm,4.6 mm×250 mm),以甲醇-0.3%冰醋酸(35∶65)为流动相,检测波长为320 nm,柱温35 ℃,流速1.0 mL/min。结果 阿魏酸在0.031~0.310 μg范围内呈线性关系(r=0.999 9),平均回收率为95.82%(RSD=0.63%)。结论 此方法操作简便、可靠,重复性好,专属性强,能有效控制该制剂的质量。
【关键词】 复方血竭凝胶;阿魏酸;高效液相色谱法;含量测定
Abstract:Objective To establish a method for determination of ferulic acid in Compound Xuejie Gels. Methods HPLC method was carried out on Zorbax SB-C18 (5 μm, 4.6 mm×250 mm), with detection wavelength at 320 nm. The mobile phase was composed of methanol-0.3% aqueous acetic acid (35∶65), the column temperature was 35 ℃ and the flow rate was 1.0 mL/min. Results The calibration curve was linear in the range of 0.031~0.310 μg (r=0.999 9). The average recovery was 95.82% (RSD=0.63%). Conclusion This method is si [1] [2] [3] [4] 下一页 |
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