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作者：罗国琼,黄斌,蒋荣珍,黄丽鑫
【摘要】 目的 建立高效液相色谱法测定大黄虫片中芍药苷含量的方法。方法 选用C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.1%磷酸溶液(14∶86)为流动相,流速1.0 mL/min,柱温30 ℃,检测波长为230 nm。结果 芍药苷在0.10～0.90 μg范围内与峰面积积分值呈良好的线性关系,r＝0.999 7,平均加样回收率为99.98%,RSD＝0.81%(n＝6)。结论 本法操作简便,准确,重复性好,精密度高,可用于大黄虫片的质量控制。
【关键词】 大黄虫片；芍药苷；高效液相色谱法；含量测定
Abstract：Objective To establish a HPLC method for determining the content of paeoniflorin in Dahuang Zhechong Tablets. Methods Stationary phase was C18 column (4.6 mm×250 mm, 5 μm), mobile phase was acetonitrice-0.1% phosphoric (14∶86), detection wavelength was 230 nm, flow rate was 1.0 mL/min and column temperature was 30 ℃. Results There was good linear relationship between the concentration of paeoniflorin and area integral value in the range of 0.10～0.90 μg, r＝0.999 7. The average recovery was 99.98% with RSD＝0.81% [1] [2] [3] [4] [5] 下一页 |
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