作者:廉宜君,李炳奇,刘红,马彦梅,陈韩英
【摘要】 目的 研究复合酶辅助超声波提取沙枣多糖的最佳工艺。方法 考察了纤维素酶和果胶酶在不同温度、不同酶量、不同酶作用时间下提取沙枣多糖最佳的方案,并且研究了超声处理对于酶法提取沙枣多糖的影响。结果 沙枣多糖提取的适宜条件是:纤维素酶1.5%、果胶酶1.5%;酶解时间为40 min,酶解温度为55 ℃,超声时间25 min,超声功率400 W,固液比1∶30,超声温度60 ℃。在此条件下,沙枣多糖的提取率可达12.35%。结论 本工艺简单可行,可用于沙枣多糖的提取。
【关键词】 沙枣;多糖;酶;超声波;提取工艺
Abstract:Objective To optimize the technological conditions of polysaccharide extraction from Elaeagnus angustifolia L. by enzymatic-assisted ultrasonic. Methods The effects of different enzymes, enzymolysis temperature, enzyme dosage, enzymolysis time, material-water ratio, crushing degree, ultrasonic time and ultrasonic temperature on the extraction rate of Elaeagnus angustifolia L. polysaccharide was analyzed. Results The optimal technological condition was as follows:1.5% cellulase, 1.5% pectic enzyme, enzymolysis time 40 min, enzymolysi
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