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华支睾吸虫乳酸脱氢酶E10-20及E94-102表位的克隆表达与生物学特性初步研究 |
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华支睾吸虫乳酸脱氢酶E10-20及E94-102表位的克隆表达与生物学特性初步研究 |
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94-102 were cloned to pGEX-4T-1 vector, then the recombinant protein were expressed and purified. CsLDH immunized sera was used as the first antibody, two epitopes were identified by Western blotting and IgG-ELISA analysis. E10-20 and E94-102 immunized sera was used as the first antibody, CsLDH was identified by the same methods above. Enzyme activity of recombinant CsLDH was assayed in the standard reaction system by adding different dilution sera. 【Results】 The recombinant plasmids pGEX-4T-1-E10-20 and pGEX-4T-1-E94-102 were constructed successfully. The expression products and purified ones were identified by SDS-PAGE. Western blotting and ELISA both showed that the CsLDH immunized sera could identify the two epitopes and E94-102 more easily. Epitopes immunized sera both could identify CsLDH by Western blotting and ELISA analysis. E94-102 immunized sera could inhibit the CsLDH enzyme activity as the CsLDH immunized sera could do. 【Conclusion】 Construction and function of the two epi 上一页 [1] [2] [3] [4] 下一页 |
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