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肠激安方对腹泻型肠易激综合征模型大鼠T淋巴细胞和5羟色胺的影响 |
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| 肠激安方对腹泻型肠易激综合征模型大鼠T淋巴细胞和5羟色胺的影响 |
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作者：唐洪梅，黄樱华，李得堂,丘振文，阎雪
【摘要】 【目的】观察肠激安方对腹泻型肠易激综合征（IBS）模型大鼠T淋巴细胞水平和5羟色胺（5HT）分泌的影响。【方法】选用新生SD大鼠46只，随机分成正常组，模型组，肠激安方低、中、高剂量组（中药低、中、高剂量组，剂量分别为094、188、376g·kg-1·d-1），补脾益肠丸组（剂量为676g·kg-1·d-1）；采用直肠内醋酸刺激法复制肠道高敏感大鼠模型，经大鼠排便统计及肠道敏感性评估确认造模成功后，采用流式细胞术检测各组外周血CD+3、CD+4、CD+8T淋巴细胞亚群水平，采用免疫组化法观察各组大鼠肠组织5HT阳性细胞数和免疫染色强度。【结果】模型组大鼠排便量显著增加，肠道敏感性显著升高，CD+4水平及CD+4/CD+8比值显著降低，CD+8水平显著升高，5HT阳性细胞数及染色强度显著增加（均P＜001）；中药低、中、高剂量组可减少大鼠排便量（治疗第14天），降低肠道敏感性，升高CD+4水平及CD+4/CD+S比值，降低CD+8水平，减少5HT阳性细胞数及染色强度（均P＜001）。【结论】肠激安方治疗IBS的作用可能与改善细胞免疫功能和肠道敏感性，减少5HT分泌有关。
【关键词】 肠激安方/药理学；肠易激综合征/中药疗法；肠易激综合征/免疫学；结肠/病理学；疾病模型，动物；大鼠
Abstract: ObjectiveTo observe the effect of Changjian Prescription (CP) on T lymphocytes count and 5hydroxytryptamine (5HT) secretion in rats with diarrhea predominant irritable bowel syndrome (IBS). MethodsFortysix neonatal SD rats were randomized into normal group, model group, low, middle and highdose CP groups (094, 188 and 376g·kg-1·d-1 respectively), and Bupi Yifei Pills group (676g·kg-1·d-1). Continuous internal rectal stimulation with acetate was used to induce the rat model of intestinal hypersensitive IBS. Flow cytometer was used for the quantitative analysis of peripheral levels of T lymphocytes subsets CD+3 , CD+4 and CD+8 , and immunohistochemical method was used to observe 5HTpositive cells number and the immunostaining intensity in the bowels of rats .ResultsIn the model group, the defecation volume and the intestinal sensitivity were increased, CD+4 level and CD+4/CD+8 decreased, CD+8 level increased, 5HTpositive cells number and the immunostaining intensity elevated (P＜001 compared with the normal group). The defecation volume 14 days after treatment and the intestinal sensitivity were decreased, CD+4 level and CD+4/CD+8 increased, CD+8 level [1] [2] [3] [4] [5] 下一页 |
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