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曲古抑菌素A对膀胱癌T24细胞增殖及Apaf-1和APC基因表达的影响 |
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| 曲古抑菌素A对膀胱癌T24细胞增殖及Apaf-1和APC基因表达的影响 |
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optosis changes and the cell cycle distribution were examined by flow cytometry (FCM). The expression of Apaf-1 and APC mRNA was detected by RT-PCR. 【Results】 TSA significantly inhibited the proliferation of T24 cells; Flow cytometry showed that the cells in G0/G1 phase and the apoptotic rates were significantly increased after treatment with TSA， while the cells in S phase were reduced.RT-PCR results showed that the Apaf-1 and APC mRNA level were promoted after TSA treatment. 【Conclusion】 TSA can inhibit T24 cells growth in vitro through inducing cell apoptosis and cell cycle arrest， which might be related to the expression of Apaf-1 and APC.
组蛋白乙酰化修饰是表观遗传调控最主要的研究内容之一，其通过改变染色质的构型调控基因转录，与恶性肿瘤具有密切的关系。抑制细胞内组蛋白去乙酰化酶(histone deacetylase， HDAC)的活性，可增加组蛋白的乙酰化程度，具有抑制肿瘤细胞的生长，诱导分化和(或)凋亡的作用[1]。曲古抑菌素(trichostatin A，TSA)是一种氧肟酸类HDAC抑制剂，本研究应用TSA体外作用于人膀胱癌T24细胞株，观察其细胞生物学行为改变和人凋亡蛋白酶活化因子-1基因(apoptosis protease activating factor-1，Apaf-1)、结肠腺瘤性息肉病基因(adenomatous polyposis coli，APC)表达的变化，探讨TSA作用于 上一页 [1] [2] [3] [4] 下一页 |
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