Abstract： 【Objective】 To evaluate the impact of serum estradiol (E2) and other hormone levels after human chorionic gonadotropin (HCG) administration on IVF-ET outcome during controlled ovarian hyperstimulation (COH). 【Methods】 In this prospective study， blood samples were collected from a total of 76 women with sterility undergoing COH for IVF-ET to assay the hormones on the day of ovarian stimulation initiation， HCG administration， ovum picking-up (OPU) and embryo transferring (ET). Patients were divided into two groups： pregnant group (n = 28) and non-pregnant group (n = 46). 【Results】 The E2 [(2 380 ± 1 177 vs. 839 ± 446 vs. 1 199 ± 634) pg/mL， P < 0.01]， progesterone (P) [(0.50 ± 0.29 vs. 7 ± 4 vs. 84 ± 48) ng/mL，P < 0.01] levels on the day of HCG administration， OPU and ET were significantly different in the 74 patients. The number of oocytes retrieved were positively correlated with the E2 levels at the day of HCG administration (r =

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