| 改良组织块法培养细针活检滑膜组织成纤维样滑膜细胞 |
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re and modified tissue culture). Cell count and the percentage of viable cells were observed by trypan blue staining. FLS were identified by morphology， immunocytochemical staining and flow cytometry. 【Results】 All three methods could culture FLS successfully，of which modified tissue culture method was better than the other two methods. FLS cell count was (8.30 ± 1.65) × 105 per flask with more than 95% viable cells by Trypan blue staining. FLS of the third or forth generation showed typical morphological characters under inverted phase contrast microscope and transmission electron microscope with Vimentin+/CD68-/CK+ staining and (95.34 ± 2.47)% CD55+ cells. Modified tissue culture method has higher successful rate with earlier and more quickly cell emigration from synovium tissue. 【Conclusion】 Modified tissue culture method is especially suitable for FLS culture in vitro from needle biopsied synovium tissue. The cell count， the percentage of viable cells， and purity of FLS of the thir 上一页 [1] [2] [3] [4] 下一页 |
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