作者：何金鑫 宋芳 崔成立 李斌

【摘要】  目的：观察比较10%甲醛、丙酮和Bouin三种固定液对神经纤维的固定效果，为临床做出更好的神经纤维的快速冰冻切片提供实验基础。方法：取健康大鼠双侧坐骨神经，冰冻切片后分别用三种固定液固定，然后做ＨＥ染色，观察各自的组织结构及染色情况。结果：１０%甲醛固定的神经纤维组织结构较清楚但是组织发生收缩；丙酮固定的神经纤维染色效果较好，组织没有发生收缩；Bouin固定液固定的神经纤维结构和染色都比较好但是组织收缩最明显。结论：三种固定液都可用于固定神经纤维，但丙酮效果最好。

【关键词】  固定液；冰冻切片；HE染色
   
　　Abstract  Objective:To compare the fixation effects of nerve fibers by three fixatives, 10% formaldehyde, acetone, and bouin so as to provide experimental proof for making rapid frozen section of nerve fibers clinically. Methods: Sciatic nerves dissected from healthy rat were subjected to the technique of frozen section and then followed by fixation, and HE staining. The tissue structure of each HE-stained sciatic nerve frozen section was carefully examined. Results: Sciatic nerve frozen section fixed

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