多孔磷酸钙生物材料的制备及表征 |
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【摘要】 目的 制备适合新骨长入的孔隙率、孔隙尺寸和结构的多孔磷酸钙组织工程支架材料。方法 通过湿法共沉淀法合成羟基磷灰石(HA)/磷酸三钙(TCP)双相粉末,采用有机泡沫浸浆法和适当的致孔剂,分别制备多孔磷酸钙陶瓷和多孔磷酸钙骨水泥。采用扫描电镜观测试样的孔隙结构,测量其孔隙率,利用X射线衍射分析其相成分。结果 多孔磷酸钙陶瓷和磷酸钙骨水泥的孔隙率分别为72%和67%;孔隙尺寸分布在200μm和280μm左右;孔壁上分别存在大量孔径为数微米至数十微米的微孔;X射线衍射结果证明多孔磷酸钙陶瓷的组成包括HA和βTCP ,骨水泥的成分为HA,αTCP and βTCP。结论 试验制备的多孔磷酸钙生物材料具有适合新骨长入的孔隙直径、孔隙率和良好的生物相容性。
【关键词】 多孔磷酸钙陶瓷; 骨水泥; 微孔; 生物相容性
Preparation and Characteration of Porous Calcium Phosphate Bioactive Material for Bone Tissue Engineering
【Abstract】 Objective To prepare the adaptive porous calcium phosphate bioactive material. Methods The hydroxyapatite/tricalcium phosphate biphasic powder was synthesized by the wet coprecipitation method. Then polymer sponge immersion method and appropriate progeny were used to prepare porous calcium phosphate (CaP) .The morphology and structure of CaP ceramics and CPC were observed by scanning electronic microscopy (SEM).Their phase composition were analyzed by Xray diffraction(XRD). Results The porosities of CaP ceramics and CPC were 72% and 67% respectively. Their pore sizes were 200 to 280 μm. The results of XRD showed that CaP ceramics composed of HA and βTcp, CPC composed of HA, βTCP. Conclusion The preparation by the experiment has appropriate pore size and porosity and excellent biocompatibility which are suitable to the bone ingrowth.
【Key words】 porous calcium phosphate (CaP) ceramics; cement; micropore; biocompatibility
1 前 言
磷酸钙生物材料由于具有与自然骨组织相似的无机成分,因此表现出优良的生物相容性,植入体内后能够与骨组织形成骨键合,被称为生物或材料,并已经成功地被应用于临床骨缺损的修复和填充[1]。但是,临床常常会遇到病理情况下的骨缺损,如骨质疏松导致的骨缺损,患者的骨密度低,骨修复能力差;或者大体积的骨缺损。由于无机的磷酸钙生物材料缺乏生长因子、蛋白质等,引导新骨长入磷酸钙生物材料的能力有限,上述原因常常导致临床不愈合或延迟愈合。因此,有研究提出通过体外培养细胞或在磷酸钙生物材料中添加一定的生物因子,如骨形态发生蛋白[2](BMP),转移生长因子[3](TGFβ)等,或者在生物材料体外培养细胞,通过添加生长因子或细胞赋予生物材料诱导组织再生 [1] [2] [3] [4] 下一页 |
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