| 广藿香愈伤组织诱导和分化再生植株的研究 |
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作者：林小桦，贺红，吴立蓉，张燕玲，刘星
【摘要】 【目的】开展广藿香愈伤组织的诱导及分化再生植株的研究，建立高效的组织培养再生系统。【方法】以广藿香叶片、带节茎、不带节茎及根尖为材料进行离体培养，筛选影响愈伤组织诱导、增殖及分化的因素。【结果】6苄氨基嘌呤(BA)或N苯基N123噻二唑5脲(TDZ)与α萘乙酸(NAA)配合有利于愈伤组织的诱导，BA的适宜浓度为02～05mg/L；4种外植体中，带节茎及不带节茎较易诱导出愈伤组织。BA和NAA配合使用能较好地促进愈伤组织的增殖；培养30d的愈伤组织较60d的具有更强的增殖和分化能力。BA有利于愈伤组织的分化；叶片诱导的愈伤组织分化能力较强。【结论】建立了高效的广藿香愈伤组织诱导及分化再生植株的离体再生体系。
【关键词】 广藿香/生长和发育；离体培养；愈伤组织
Abstract: ObjectiveCallus induction and plantlets regeneration of Pogostemon cablin(Blanco)Benth. are studied to establish a highefficiency regeneration system for tissue culture.MethodsThe leaf segments, nodular stem segments, stem segments and root tips from Pogostemon cablin(Blanco)Benth. were cultured in vitro. The factors which affect the induction，multiplication and differentiation of callus were studied. ResultsBenzylaminopurine (BA) or thidiazuron (TDZ) combined with αnaphthylacetic acid (NAA) was helpful to induce callus. The optimal concentrations of BA were 02～05mg/L. Nodular stem segments and stem segments were the most suitable explants for callus induction. The medium containing BA and NAA promoted the multiplication of callus. The multiplication rate and differentiation rate of 30day callus were much higher than those of 60day callus. BA was helpful to induce callus differentiation. The callus from leaf segments exerted a higher differentiation rate. ConclusionA highefficiency regeneration system of callus induction and plant regeneration from Pogostemon cablin(Blanco)Benth.has been established.
Key words:POGOSTEMON CABLIN(BLANCO) BENTH./growth & development;INVITRO CULTURE;CALLUS
广藿香［Pogostemon cablin (Blanco) Benth.］为唇形科刺蕊草属植物，以干燥地上部分入药，其性辛、微温。具有芳香化湿，开胃止呕，发表解暑的功效［1］。广藿香为广东道地药材，“十大广药”之一，是多种著名中成药“藿香正气丸”、“藿胆丸”等以及现代中药制剂“抗病毒口服液”的主要原料。广藿香原产于菲律宾、马来西亚等国，最早自宋代引入我国，栽培历史悠 [1] [2] [3] [4] [5] 下一页 |
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