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本文由中国论文联盟医学院第一附属 医院 骨科椎间盘突出症患者术后椎间盘病理组织34例用于实验组,年龄27~69岁,平均年龄46.24±11.25岁;取脊柱骨折外伤术后椎间盘组织标本7例,并取石河子大学医学院病理科尸检椎间盘标本8例,共15例用于对照组,年龄25~52岁,平均年龄40.07±8.43岁。采用免疫组化法和ELISA两种方法进行检测。[结果]光镜下观察,免疫组化检测TNF-α、IL-18蛋白表达阳性细胞表现为细胞浆中出现棕黄色、褐色或浅棕黄色染色,结果判定及统计学分析后表明:实验组TNF-α蛋白表达高于对照组,差异有统计学意义(P<0.01),实验组IL-18蛋白表达高于对照组,差异有统计学意义(P<0.01)。Elisa法证实实验组中TNF-α含量为82.18±21.91 ng/ml,IL-18的含量为45.39±21.23 ng/ml;对照组中TNF-α含量为8.68±0.78 ng/ml、IL-18含量为3.58±2.84 ng/ml。两者差异均有统计学意义(P<0.01),且两种细胞因子的表达均与患者年龄及病程呈正相关。[结论]椎间盘组织中细胞因子TNF-α及IL-18表达与椎间盘退变有关,推测可能是导致椎间盘组织退变的重要原因之一。
【关键词】 肿瘤坏死因子-α; 白介素-18; 椎间盘突出症; 免疫组织化学; Elisa法
Abstract: [Objective]To detect the expression of tumor necrosis factor-α(TNF-α )and interleukin-18(IL-18)protein in herniated intervertebral disc,and discuss the role of TNF-α and IL-18 protein in the course of the intervertebral disc degeneration and to clarify the mechanism of the lumbar intervertebral disc degeneration and herniation. [Method]Forty nine specimens were studied from December 2005 to April 2007 in the First Affiliated Hospital of Shihezi Medical College.Experimental group contained thirty-four herniated intervertebral disc and control group contained fifteen discs,which were collected from the patients with spine fracture or autopsy. The specimens were divided into two parts,one part was fixed in 10% formalin about 24 hours and was embedded in paraffin.Four μm serial sections and S-P method were used.The sections were stained with TNF-α and IL-18 monoclonal antibodies,and the results were observed by OLYMPUS microscope.Another part was changed into well-distributed liquid,which was determined by Elisa method respectively,in order to detect density of TNF-α and IL-18 protein. Software SPSS 13.0 was used for statistical analysis.[Result]1 TNF-α and IL-18 protein were expressed mostly in cytoplasma.The expression of IL-18 in the experiment group was obviously higher than in the control group.There were statistical significances(P<0.01).The expression of TNF-α in the experiment group was obviously higher than in the control group.There was statistical significance(P<0.01).2 The method of Elisa confirmed that TNF-α and IL-18 contents in the experiment group were markedly higher than the control group. There was statistical significance(P<0.01).[Conclusion]There is obvious link between the expression of TNF-α and IL-18 proteins in herniated intervertebral disc and disc degeneration. The increase of the expression of TNF-α and IL- [1] [2] [3] [4] 下一页 |
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