胃癌中微淋巴管密度检测的意义

【摘要】 目的利用淋巴管内皮生长因子受体3(vegfr3)检测胃癌组织内微淋巴管密度(mld)，研究其与增殖期细胞核抗原(pcna) 及临床因素之间的相关性。方法采用免疫组化法，以vegfr3 (又称flt4) 为标记物，检测60例胃癌淋巴结转移组织内mld与增殖期细胞核抗原(pcna) 蛋白及临床病理因素之间的相关性。结果胃癌淋巴结转移组织vegfr3阳性率和mld明显增高;胃癌组织中mld与pcna之间呈正相关。结论 vegfr3可促进肿瘤微淋巴管生成,vegfr3和mld的表达可能与胃癌的淋巴结转移相关。

【关键词】 胃癌;淋巴管内皮生长因子受体3;微淋巴密度

abstract：objective to detect the lymphatic microvessel density(mld) in gastric cancer by lymphatic endothelial growth factor receptor3(vegfr3) and to study the correlation between the proliferating cell nuclear antigen(pcna) protein and clinicopathological factors.methods vegfr3 was used as a marker to detect the expression of mld and pcna protein in 60 cases of gastric cancer tissues by immunohistochemical method.results the positive rate of vegfr3 and mld were significantly increased in gastric cancer with lymph node metastasis,and there was a positive correlation between.mld and pcna in gastric cancer.conclusion vegfr3 might promote microtumor lymphangiogenesis,and vegfr3 and the expression of mld may be related to lymph node metastasis of gastric cancer.

key words：gastric cancer;lymphatic endothelial growth factor receptor 3;lymphatic microvessel density

肿瘤的侵袭和转移是患者死亡的重要原因之一。LOcAlHost胃癌早期以淋巴转移为主，晚期才发生血行转移。尽管淋巴管在胃癌转移中的作用非常明确，但因长期未发现明确的淋巴管内皮标记物和淋巴管生成因子，使人们对胃癌淋巴管生成(lymphangiogenesis)的研究相对滞后于胃癌血管生成(angiogenesis)。淋巴管内皮生长因子受体3(vascular endothelial growth factor receptor3，vegfr3)是vegf基因家族成员vegfc的受体，主要在淋巴管内皮细胞和单核细胞系造血细胞中表达。因此，vegfr3可作为淋巴管内皮细胞的标记物，以区别于血管内皮细胞[1,2]。本实验采用免疫组化法和图片分析法，检测胃癌组织中vegfr3和微淋巴管密度(microlymphatic density，mld)的表达并探讨其与增殖期细胞核抗原( proliferating cell nuclear antigen，pcna)及临床病理因素之间的相关性。

1 材料与方法

1.1 标本来源

选择1999～2002年在咸宁学院附属第一
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