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生长激素上调性激素抑制下青春期大鼠生长板原代软骨细胞表皮生长因子的表达 |
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| 生长激素上调性激素抑制下青春期大鼠生长板原代软骨细胞表皮生长因子的表达 |
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wth factor (EGF) synthesis (EGF peptide and EGF mRNA levels) in cultured growth plate chondrocytes. 【Methods】 At 3 week of age， Sprague-Dawley rats received 2.5 mg/kg in slow-released GnRHa (triptorelin) which was repeated every 2 weeks for 2 times till 7-week old. Chondrocytes from tibial growth plate of 5 ～ 8 Sprague-Dawley rats by trypsin-collagenase digestion were cultured in monolayer. Primary chondrocytes were divided into time?鄄effect groups and concentration-effect groups. MTT and immunohistochemical staining of proliferating cell nuclear antigen (PCNA) were conducted to observe the effect of GH on the proliferation of chondrocytes. RT-PCR and ELISA method were conducted to detect EGF expression in mRNA level and protein level respectively. 【Results】 GH enhanced the proliferation of the chondrocytes in time-course-dependent and concentration-dependent manner. Incubation with GH 100 ng/mL maximally increased expression levels of EGF mRNA (for 18 h) and EGF peptide (for 36 h) res 上一页 [1] [2] [3] [4] [5] [6] 下一页 |
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