| 小麦成熟胚离体培养研究 |
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论文联盟*编辑。 摘要：以美国小麦品种Overley成熟胚作为外植体进行离体培养，研究外源激素对小麦成熟胚诱导愈伤组织、分化出苗以及试管苗增殖的影响。试验结果表明，在MS培养基中添加2,4-D 3 mg·L-1愈伤组织诱导频率较高，成愈率为84.0%；在添加有KT 2~3 mg·L-1的MS分化培养基中愈伤组织分化率较高，可达85%以上；将试管苗转接至添加0.2 mg·L-1的NAA和0.5~2.0 mg·L-1的6-BA的培养基中，试管苗有一定的分化增殖，但最高分化率仅为33.3%，增殖系数2.05。
关键词：小麦；成熟胚；离体培养；植株再生
Study on Mature Wheat Embryos Culture in Vitro
SUN Ning1, ZHAO Xin-hai2, CHEN Xiao-qiang1, ZHANG LEi1
（1.Department of Agronomy, Tianjin Agricultural University, Tianjin 300384,China；2. Department of Computer Science and Information Engineering，Tianjin Agricultural University，Tianjin 300384， China）
Abstract：Mature wheat embryos of Overley were used as explants for culture in vitro. The result showed that the optimum medium for callus induction was MS+2,4-D（3.0 mg·L-1）, the rate of callus formation was 84.0%. The medium for callus differentiation was MS+KT（2~3 mg·L-1）, the differentiation of callus was more than 85%. The wheat plantl[1] [2] [3] [4] [5] [6] 下一页 |
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