| 温度和培养基在申克孢子丝菌酵母相转化中的作用研究 |
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BHI， 50 mg/kg goat blood BHI and 5 mg/kg GSBHI， and 5 plates of each media were prepared because isolates of each meida were incubated at 25 ℃， 35 ℃， 36 ℃， 37 ℃， and 38 ℃. Growth of Sporothrix schenckii was observed and rates of transformation were calculated. 【Results】 At 38 ℃， the transformations rates were 25%(the only one Strain converted yeast). At 35 ℃ and 37 ℃， the transformations rates were 60% and 85%， respectively. At 36 ℃， the transformations rates were 45%. There were 13 of 20 plates converting yeast when 5% goat blood BHI and 0.5%GSBHI were used， 9 of 20 plates when BHI was used， 4 of 20 plates when SDA and PDA were used. At 37 ℃， transformation rates of 3 isolates reached 90% on medium of BHI， 50 mg/kg goat blood BHI和5 mg/kg GSBHI; However， the transformation rates of 1 isolate were less than 10% on SDA， PDA， and BHI. 【Conclusion】 Temperature is the key factor that affects mycelium-to-yeast transformation of Sporothrix schenckii， nourishments of media may also be necessa 上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] 下一页 |
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