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EMB基因330密码子分子DNA探针设计及荧光检测 |
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| EMB基因330密码子分子DNA探针设计及荧光检测 |
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lar DNA probe detecting embB306 codon and detecting fluorescence signal from hybridization between the amplified product and probe by fluorescence spectrophotometer，and confer to the sequencing results. Results The difference between PCR products from standard strain and Ethambutol resistant one is obvious in detecting the fluorescent light by use of fluorescence microscope. We detected fluorescent light signal between the 33 Ethambutol resistant strains and 10 H37RV standard strains. The rate of resistant Ethambutol detected is about 3%，and the rate of sequencing is about 3%. Conclusion The technology of molecular DNA probe can effectually detect a mutation single basyl site of embB 330 codon. Fluorescence spectrophotometer have characteristics such as high sensitiveness，simple to directly detect the fluorescent light in liquid.
Keywords: Ethambutol resistant embB gene; 330 codon of mutation site; molecular DNA probe; fluorescence spectroph 上一页 [1] [2] [3] [4] [5] [6] [7] 下一页 |
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