| 基于发光金纳米粒子荧光增强法测定溶菌酶 |
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say is very important. Here, water-soluble BSA-modified fluorescent gold nanoparticles （GNPs） were prepared according to the literature, and their interaction with lysozyme was investigated. A novel fluorescence method for the determination of lysozyme has been developed based on the enhancement effect of lysozyme on the fluorescence of GNPs. The effects of GNPs concentration, pH, reaction time and coexisting substances on the determination were tested. Under the optimum conditions, i.e. 4.0 ×10－6 mol/L GNPs, pH 7.0, and reaction time of 10 min, the linear range of the calibration curve was from 2×10－7 mol/L to 8×10－6 mol/L and the detection limit was 3.0×10－8 mol /L. The relative standard deviation was 2.6% for six replicate measurements of a solution containing 4.0×10－6 mol/L lysozyme. The method bore the merits of high sensitivity, good water-solubility and low-toxicity of probe. Meanwhile, some common proteins with low isoelectric poin 上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] ... 下一页 >> |
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