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钙整合素结合蛋白CIB的原核表达及多克隆抗体的制备与亚细胞定位 |
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| 钙整合素结合蛋白CIB的原核表达及多克隆抗体的制备与亚细胞定位 |
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or pET32aCIB. The CIB fusion protein was expressed in BL21 (DE3)/pET system and identified by SDSPAGE. The mice were immunized with the polyacrylamide gel particles containing the CIB fusion protein for polyclonal antibody preparation. The antibody was purified by affinity chromatographic column matrix coupled with protein G, antigen respectively and then identified by Western blot and immunohistochemistry. RESULTS: The protein of CIB was obtained by recombination expression. The specificity of polyclonal antibody was obtained by immunizing BALB/c mice with polyacrylamide gel particles containing the fusion protein of CIB and purification. The results of immunohistochemistry demonstrated that CIB was localized predominantly in the cytoplasm of SHG44 and Hhu7 cells. CONCLUSION: The protein of CIB has been cloned and expressed successfully. The specific polyclonal antibody against the protein of CIB has been obtained, which can be used fo 上一页 [1] [2] [3] [4] [5] [6] [7] [8] 下一页 |
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