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人大肠癌抗原基因 cDNA噬菌体表达文库的构建和鉴定 |
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| 人大肠癌抗原基因 cDNA噬菌体表达文库的构建和鉴定 |
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ules（<400 bp）were removed through size fraction. After the cDNAs were inserted into ZAP expression vector, the ligated products were packaged in vitro and the bacteriophage particles infected the host strains XL1Blue MRF′. RESULTS: The efficiency of the primary was 2.3×109 pfu/L, while the recombination rate reached to 97%. The average length of the inserted fragment was over 1 kb. CONCLUSION: The quality of colorectal carcinoma cDNA phage expression library is excellent and helpful to screen colorectal carcinoma specificantigens.
［Keywords］colorectal carcinoma; bacteriophage; cDNA expression library
大肠癌是消化系统的常见恶性肿瘤之一, 死亡率有逐年上升的趋势。因此早期诊断和治疗是提高大肠癌治愈率的关键［1］。我们已成功制备了以人大肠癌细胞系CCL187为免疫源的鼠抗人大肠癌单克隆抗体(mAb) ND1［2］, 进一步筛选和鉴定ND1 mAb所识别的特异性大肠癌抗原, 可为大肠癌早期诊断和免疫治疗提供新的靶点。而寻找抗原基因的最有效方法是构建cDNA文库 , 继而进行筛选、 克隆。国内外已开展大肠癌cDNA文库构建和大肠癌相关肿瘤抗原筛选方面的研究［3, 4］。本实验中用2株人大肠癌细胞系CCL187和CX1作为构建文库的材料来源, 以λ ZAP Express为载体, 构建了人大肠癌抗原基因的 上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] 下一页 |
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